This is a study on Azotobacter beijerinckii and the effects of oxygen levels on its respiration, PHB production, and other parameters.
They varied the oxygen in the inflowing gas between 0 and atmospheric (20%), and also varied dilution rates (when not holding D at 0.1 h-1).
What They Saw
As oxygen decreased, the PHB content of cells increased up to about 50% of the dry weight near 0% oxygen. Activities of PHB-producing enzymes increased also, while glucose-6-phosphate dehydrogenase activity remained constant. Below about 5% oxygen, the culture seemed oxygen-limited (no dissolved oxygen was detectable). They also saw increased respiratory activity and carbon dioxide production as oxygen increased, and indication of respiratory protection of nitrogenase.
When they increased the dilution rates (from 0.1 up to 0.2), they saw an increase in the activity of each enzyme tested. PHB content decreased as D increased, which is inconsistent with the increase in PHB enzyme activities, but it's likely that enzymes breaking down PHB were more active too.
The Entner-Doudoroff pathways seems to be the main glucose metabolism pathway in A. beijerinckii.
Reference:
Carter, I. S. & Dawes, E. A. Effect of Oxygen Concentration and Growth Rate on Glucose Metabolism, Poly-β-Hydroxybutyrate Biosynthesis and Respiration of Azotobacter beijerinckii. J. Gen. Microbiol. 110, 393–400 (1979).
They varied the oxygen in the inflowing gas between 0 and atmospheric (20%), and also varied dilution rates (when not holding D at 0.1 h-1).
What They Saw
As oxygen decreased, the PHB content of cells increased up to about 50% of the dry weight near 0% oxygen. Activities of PHB-producing enzymes increased also, while glucose-6-phosphate dehydrogenase activity remained constant. Below about 5% oxygen, the culture seemed oxygen-limited (no dissolved oxygen was detectable). They also saw increased respiratory activity and carbon dioxide production as oxygen increased, and indication of respiratory protection of nitrogenase.
When they increased the dilution rates (from 0.1 up to 0.2), they saw an increase in the activity of each enzyme tested. PHB content decreased as D increased, which is inconsistent with the increase in PHB enzyme activities, but it's likely that enzymes breaking down PHB were more active too.
The Entner-Doudoroff pathways seems to be the main glucose metabolism pathway in A. beijerinckii.
Reference:
Carter, I. S. & Dawes, E. A. Effect of Oxygen Concentration and Growth Rate on Glucose Metabolism, Poly-β-Hydroxybutyrate Biosynthesis and Respiration of Azotobacter beijerinckii. J. Gen. Microbiol. 110, 393–400 (1979).
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