Friday, August 28, 2015

663 - Azotobacter vinelandii Vanadium Nitrogenase: Formaldehyde Is a Product of Catalyzed HCN Reduction, and Excess Ammonia Arises Directly from Catalyzed Azide Reduction

The V nitrogenase is similar to but distinct from the Mo nitrogenase in various ways. This study tests its activity and patterns with cyanide and azide as substrates; previously this had only been tested with the Mo nitrogenase.

What They Saw
As with the Mo version, cyanide inhibited hydrogen production and the overall electron flux through the V nitrogenase, though less than with the Mo nitrogenase; more cyanide had more effect, up to 75% inhibition of hydrogen and electrons at 50 mM cyanide. Methane formation from cyanide increased at first, and then decreased with more cyanide, while ammonia continued to increase. They didn't measure methylamine in most conditions though, so the electron flux numbers might be off. With low cyanide, there was significant methylamine relative to the methane, about 0.66 to 1, higher than the Mo nitrogenase; this ratio seems to increase to 1:1 as methane decreases.

Interestingly, the enzyme also produces formaldehyde from cyanide. It's not clear whether the Mo version does too and it's too hard to detect, or if it just doesn't. It can be tricky, since other components in the reaction react with it, and cyanide inhibits the Mo enzyme a lot more.

Azide inhibited hydrogen production from the V nitrogenase about 50%. Hydrazine was more of a product from azide than it is for the Mo nitrogenase, relative to dinitrogen and ammonia. Overall activity was less than the Mo version, which is typical, but also because azide seems to reduce the total electron flux in this version. The ammonia seems to come from azide directly, not from the dinitrogen produced, because adding hydrogen gas (which specifically inhibits nitrogen reduction) didn't change the values.

They tried seeing what adding carbon monoxide (CO) might do to affect these reactions. With azide, it rescued hydrogen production, though not the electron flux, and CO didn't entirely prevent some azide reduction. With cyanide, the effects were similar, except the electron flux inhibition was relieved too.

What This Means
It seems that these chemicals have similar effects on the V nitrogenase as they do on the Mo version, but not completely the same. I wonder, though, about the CO assays: CO can be a substrate for the V nitrogenase, reduced mostly to ethylene but also some propylene and methane; how did this affect their assays?

Reference:

Thursday, August 27, 2015

615 - ATP-dependent reduction of azide and HCN by N2-fixing enzymes of Azotobacter vinelandii and Clostridium pasteurianum

This study again looks at nitrogenase in Azotobacter vinelandii and also Clostridium pasteurianum and how it affects/is affected by stuff like azide and cyanide.

What They Saw
They were looking at cell extracts, protein activity in vitro. Nitrogenase can reduce azide to ammonia (and other stuff); carbon monoxide can inhibit this process completely, and nitrous oxide (N2O) partially. More ammonia is formed from azide than from dinitrogen even. However, each azide molecule is reduced to one N2 and one NH3, so two thirds of the nitrogen becomes gas instead of being fixed. Presumably if left long enough, all the N2 would become ammonia though.

In terms of amount of hydrogen produced with azide present, it varied from between 28 and 35% of what was produced with no substrate present, similar to dinitrogen.

With cyanide, the enzyme reduces it to methane, ammonia, and methylamine (CH3NH2). CO inhibits this reaction completely, and azide and nitrous oxide partially. When nitrogen gas and cyanide are present, less base is formed than when only nitrogen is present, so there is competition.

In terms of hydrogen again, with cyanide there was 17% as much hydrogen as with no substrate, but it also seemed like cyanide reduced the electron flux to the enzyme overall, to about 30% of when fixing nitrogen. If CO was added with cyanide, it restored hydrogen production up to 58% of what it was with neither. This is pretty consistent with previous results.

They also tried methylamine reduction directly, but it was a very poor substrate. The same was true of cyanate (NCO-), and they didn't detect any reduction of CO to methane. (Though they maybe should've looked for ethylene, since the Mo nitrogenase produces mostly that and none of methane from CO).

What This Means
So nitrogenase can reduce a bunch of things pretty well: nitrogen, nitrous oxide, azide, cyanide, and acetylene. It's an interesting enzyme.

This study has a good summary table of different substrates for nitrogenase, products made from them, and their effects on hydrogen production/electron flux.

Reference:

Tuesday, August 25, 2015

630 - Diastereomer-dependent substrate reduction properties of a dinitrogenase containing 1-fluorohomocitrate in the iron-molybdenum cofactor

The normal nitrogenase central cofactor contains homocitrate near the central metal atom; it is required for formation of the cofactor. This study looks at compounds similar to homocitrate (diastereomers of fluorohomocitrate) incorporated into the cofactor, and how they affect the enzyme's activity.

What They Saw
They purified enzyme from Klebsiella pneumoniae and Azotobacter vinelandii I think; it's hard to tell because this paper (and others they cite) are really badly written with regard to methods. (They seriously put in a footnote saying "The experimental details of the synthesis of 1-fluorohomocitrate may be obtained from [author initials]." How is that acceptable, especially in a PNAS paper?). Somehow they got FeMo-cofactor with different analogs of homocitrate, and tested their activity with different substrates.

With threo-fluorohomocitrate, there was hardly any nitrogen fixation, similar to with citrate, but with erythro-fluorohomocitrate, there was about 3.5x less than with plain homocitrate, so it was somewhat active. Both were about half as good at acetylene reduction (measured by ethylene), and just as good at cyanide reduction (measured by methane).

In terms of hydrogen production, if there was a difference between plain homocitrate and the fluoros, it wasn't very big; they produced almost as much as the normal one. The same was true of other analogs (homoisocitrate, isocitrate, etc), though some were impaired (such as citrate, producing only about half as much).

The addition of inhibitors, carbon monoxide (CO) or carbonyl sulfide (COS), affected these different enzymes differently. The amount of hydrogen from homocitrate increased (maybe significantly) but decreased some or none for analogs, depending on the analog, up to 63%. The inhibitors reduced acetylene reduction from 35-100% in all cases, and cyanide reduction a little or a lot too.

Cyanide itself affected hydrogen production. (Dang, this paper is so badly written, it's giving me a headache trying to figure it out.) With all tested analogs (and homocitrate), cyanide inhibited hydrogen production 85-95%. Adding CO with cyanide prevented this inhibition with homocitrate and partially with fluorohomocitrate, but not much the other analogs. CO also inhibits cyanide reduction, at least with some analogs (especially the more active ones).

What This Means
This study can tell a lot about the biochemistry of different substrates binding to the enzyme and the enzyme acting on them. I wonder if the results are skewed somewhat because they only measured some of the products, not all possible ones (i.e. might some analog-based cofactors produce ethane from acetylene in addition to ethylene?). In terms of application, even if some of these analogs have desirable properties, it seems like it would be difficult to get them incorporated in vivo.

Reference:

Thursday, August 20, 2015

610 - A Nitrogen Pressure of 50 Atmospheres does not Prevent Evolution of Hydrogen by Nitrogenase

Nitrogenase always seems to produce at least one hydrogen molecule per nitrogen fixed when fixing nitrogen. This study looked at whether very high pressures of pure nitrogen could prevent this wasteful side reaction.

What They Saw
They purified nitrogenase from Azotobacter vinelandii and put it in a chamber with 50-51 atmospheres of pure nitrogen gas. The chamber was designed so they could start and stop the reaction by tipping the chamber to add reagents that would start or stop it, without changing the atmosphere. Then they measured pressure in the chamber and amount of hydrogen.

Even at the high pressure of nitrogen in this experiment, the amount of hydrogen produced was about one-to-one with the amount of nitrogen fixed. So it must be pretty essential to the reaction.

Reference:
Simpson, F. B. & Burris, R. H. A Nitrogen Pressure of 50 Atmospheres does not Prevent Evolution of Hydrogen by Nitrogenase. Science 224, 1095–1097 (1984).

598 - Nitrogenase-catalyzed reactions

This paper looks at a bunch of possible substrates and reactions that nitrogenase catalyzes.

What They Saw
They extracted nitrogenase from Azotobacter vinelandii. ATP was necessary for any activity, of course; 3 mM was the optimal amount in their in vitro reactions, possibly because of the amount of magnesium ions that were present (5 mM). They saw 1.6 hydrogens per nitrogen fixed, on average, a bit higher than the normal figure of 1; about 33% of the energy flux went to hydrogen (normal figure is 25%).

With azide added, 1 mole azide is made into 1 mole ammonia and 1 mole dinitrogen. Lower levels allowed production of hydrogen instead, but that wasn't investigated much. The same was true with acetylene; both seem better substrates than nitrogen.

Cyanide is reduced to methane and ammonia; optimal concentrations were 2-4 mM. At other concentrations, there are other products formed: ethylene, ethane, methylamine (CH3NH2). They don't discuss hydrogen.

However, their calculations of ATP per electron pair transferred were skewed because they didn't measure all the products (i.e. hydrogen gas).

Reference:
Hwang, J. C. & Burris, R. H. Nitrogenase-catalyzed reactions. Biochim Biophys Acta 283, 339–350 (1972).

Wednesday, August 19, 2015

571 - Oxygen effects on the nickel- and iron-containing hydrogenase from Azotobacter vinelandii

This study looks at how oxygen affects the uptake hydrogenase of Azotobacter vinelandii.

What They Saw
They grew A. vinelandii OP (aka CA) and purified its membrane-bound hydrogenase. When purified anaerobically, it was fully active with an electron acceptor other than oxygen (methylene blue or benzylviologen). Added oxygen appeared to inhibit this reduction of methylene blue, and this was noncompetitive inhibition (adding extra methylene blue didn't relieve it).

When oxygen was removed by adding an oxygen-binding protein (leghemoglobin), the inhibition was reversed and activity recovered.

They claim the membrane-associated hydrogenase in these experiments was incapable of reducing the oxygen; it's not clear if being more capable would change the results, but it seems likely.

There was also slower, irreversible inactivation, shown by adding oxygen to an assay and adding enough dithionite to consume all of it to remove any effect of reversible inhibition. Over time, the enzyme lost activity, whether aerobically or anaerobically purified. Purified enzyme lost more activity more quickly than membrane-bound. It seemed like activity was only lost when the enzymes were exposed when active, but simply activating them didn't reproduce the effect. It was a confusing assay.

They did find that adding hydrogen could provide protection from inactivation, up to almost 100% protection, but neither hydrogen nor oxygen was consumed during this process. Super weird.

Finally, carbon monoxide didn't help protect the enzyme from oxygen at all, nor did affect protection by hydrogen.

What This Means
It's interesting, but probably not that important physiologically. A. vinelandii is capable of withstanding high levels of oxygen, and such high levels are just as likely to inhibit the nitrogenase which produces the hydrogen as the hydrogenase which consumes it. It might be interesting to study whether oxygen inhibits the oxidation of added hydrogen though.

Reference:
Seefeldt, L. C. & Arp, D. J. Oxygen effects on the nickel- and iron-containing hydrogenase from Azotobacter vinelandii. Biochemistry 28, 1588–1596 (1989).

Tuesday, August 18, 2015

570 - Kinetic analysis of the interaction of nitric oxide with the membrane-associated, nickel and iron-sulfur-containing hydrogenase from Azotobacter vinelandii

This study looked at the effect of nitric oxide (NO) on Azotobacter vinelandii's uptake hydrogenase.

What They Saw
They isolated hydrogenases from cells but didn't separate them from the membrane, because that made them too sensitive to oxygen. When activated in a reducing environment and then exposed to NO, hydrogenase activity was inhibited, but this inhibition could be almost completely reversed by adding iron EDTA, which reacts with NO. The more NO, the more inhibition (relative to the no NO control).

When NO was added and the hydrogenase wasn't active, the inactivation was irreversible; the longer the exposure, the more the inhibition, but it took larger amounts of NO to get the same amount of inhibition as the reversible kind. Hydrogen or carbon monoxide didn't protect against this inactivation; hydrogen even enhanced the effect.

Reference:

Monday, August 17, 2015

569 - Hydrogen-oxidizing electron transport components in nitrogen-fixing Azotobacter vinelandii

This study looks at oxidation of hydrogen by Azotobacter vinelandii's uptake hydrogenase, and which proteins are involved in the electron transport chain.

What They Saw
They grew A. vinelandii CA fixing nitrogen, and isolated the membrane fraction from the cells. They looked at oxygen uptake, and saw that unless there were oxidizable substrates, there was no consumption of oxygen, which makes sense. Hydrogen fulfilled the requirement though, and there were two hydrogen molecules taken up for each molecule of oxygen, which makes sense: two hydrogen atoms for each atom of oxygen, to make H2O.

Using spectrophotometry, they observed peaks that occurred when components in the membrane were reduced with hydrogen, malate, or dithionite. Hydrogen affected cytochrome d (showing a peak at 627nm), b (shoulder at 559nm) and c (peak at 550), but not a (595). The other reductants affected b a lot more, and a somewhat.

With carbon monoxide added, hydrogen only reduced cytochrome d. The others showed a new peak, cytochrome o, at 417nm, but hydrogen didn't. The same seemed true with low levels of cyanide; so hydrogenase's terminal oxidase seems to be cytochrome d type. Not really sure how they prevented these inhibitors from inhibiting the hydrogenase itself, like they seem to in other studies.

Reference:
Wong, T. Y. & Maier, R. J. Hydrogen-oxidizing electron transport components in nitrogen-fixing Azotobacter vinelandii. J. Bacteriol. 159, 348–352 (1984).

Thursday, August 13, 2015

563 - Construction and Characterization of Hybrid Component 1 from V-Nitrogenase Containing FeMo Cofactor

This study looked at a purified V nitrogenase from Azotobacter vinelandii with the FeMo cofactor instead of FeVco.

What They Saw
They detected only Mo, no V, in the preparation. The activities they saw were pretty weird in some ways: the electron flux going to nitrogen was almost as much as in the Mo nitrogenase (~70%, with the rest going to hydrogen), whereas with the normal FeVco, 50% went to each; in previous studies, V nitrogenase with FeMoco couldn't fix nitrogen at all. On the other hand, with acetylene, almost all electrons went to ethylene in the Mo nitrogenase, whereas only 30-35% did in the V nitrogenase with either cofactor, while 3% went to ethane with FeVco but 10% did with FeMoco, which is consistent with previous studies. So I'm confused.

When they added carbon monoxide, there was inhibition of all nitrogenase versions, as expected.

What This Means
I think something was weird in this study, but I don't know what. It seems very questionable. However, most of the later studies citing this one either accept it without question, or confirm its results, so maybe it's not as questionable as it seems.

Also, it's worth noting that the results only show the electron flux going to each substrate, not the total electron flux, so even if most electrons go to nitrogen in the FeMoco-substituted V nitrogenase, it's still possible that it's fixing a lot less nitrogen overall than either Mo or V versions proper.

Reference:
Moore, V. G., Tittsworth, R. C. & Hales, B. J. Construction and Characterization of Hybrid Component 1 from V-Nitrogenase Containing FeMo Cofactor. J. Am. Chem. Soc. 116, 12101–12102 (1994).

Wednesday, August 12, 2015

558 - Isolation of a new vanadium-containing nitrogenase from Azotobacter vinelandii

This study is the first to fully purify the vanadium nitrogenase from Azotobacter vinelandii.

What They Saw
They used strain UW (aka CA) and a nifHDK knockout. It didn't seem like they scrubbed the medium for Mo, but they did test the purified product for metals and didn't see a detectable amount of Mo, only V and Fe (in a 1-to-13 ratio), so I guess it's good.

They did acetylene reduction assays on the V and Mo nitrogenases, and found that 90% of the electrons went to acetylene in the former, but only 12% in the latter. It seems like they only measured ethylene production though, so they might've underestimated the total activity by neglecting the ethane produced by the V nitrogenase.

Under argon, the Mo nitrogenase produced 1.6x more hydrogen than the V version. This was the same ratio as that of nitrogen fixed by each version. I'm not sure how much it's possible to compare these enzymes in vitro though. But it seems like the V nitrogenase is better at nitrogen and hydrogen than at acetylene.

As mentioned, ICP emission spectroscopy didn't detect any metals other than V or Fe: no Mo, Cr, Co, Ni, Cu, or W, so that's good.

What This Means
This shows how the Mo and V nitrogenases are similar in some ways and yet different in others. They work in similar ways and are sensitive to similar things, but the products of their reactions and such are different (though possible substrates seem largely the same). By comparing the two, we can learn more about the process of nitrogen fixation in general.

Reference:
Hales, B. J., Case, E. E., Morningstar, J. E., Dzeda, M. F. & Mauterer, L. A. Isolation of a new vanadium-containing nitrogenase from Azotobacter vinelandii. Biochemistry 25, 7251–7255 (1986).

Monday, August 10, 2015

557 - Detection of the in vivo incorporation of a metal cluster into a protein - The FeMo cofactor is inserted into the FeFe protein of the alternative nitrogenase of Rhodobacter capsulatus

This is another study looking at central cofactors from some nitrogenase versions inserted into other apoproteins than usual, but this time in Rhodobacter capsulatus.

What They Saw
They looked at purified enzymes from wild-type R. capsulatus and a nifHDK deletion mutant. The latter should only produce the iron-only nitrogenase, if anything. They had to treat the medium to remove as much Mo as possible so as to be able to control the concentration; this reduced the Mo present from around 1 ppb to less than 0.05 ppb (the detection limit).

They found that adding 10μM Mo to cultures growing with no Mo (and thus producing the Fe nitrogenase) greatly increased the amount of ethane produced from acetylene reduction (up to 40% of the ethylene produced). Without Mo, ethane remained constant at about 2% of ethylene. The amount of ethane increased over 72 hours too, up to 68% of ethylene; however, total activity decreased greatly over that time, down to only about 5% of what it had been before adding Mo. The ethane production rate increased for 24 hours and then decreased more slowly than the ethylene rate. This may be due to repression of the Fe nitrogenase, but the authors claim it is not, because the rate decreases more quickly in late-log cultures with chloramphenicol + Mo than with just chloramphenicol (or neither, which was about the same as with chloramphenicol alone). This shows that no new nitrogenase protein is being made even when chloramphenicol is absent, but adding Mo speeds the loss of it.
They also found that the more Mo they added, the higher the proportion of ethane produced (and also the lower the total acetylene reduction activity).

They tested the sensitivity of the system to oxygen, both with and without Mo: in both cases, more oxygen meant less acetylene reduction, though the system with Mo seemed a bit more sensitive (dropping to almost 0% with 1% oxygen while that without Mo only fell to about 20%), but also they noticed that increased oxygen increased the proportion of ethane produced after Mo was added. So somehow oxygen enhanced the Mo effect.

Rhenium, tungsten, and vanadium did not cause anything similar to the Mo effect. The Mo effect was also absent in mutants unable to produce FeMo cofactor (nifE knockouts), so it seems like the cofactor is part of the system. nifQ knockouts seemed to show the effect only at high concentrations of Mo (0.1-1mM); this gene's product is involved in cofactor synthesis at a different step. Mo uptake wasn't an issue; all strains had the same intracellular concentrations.

Using EPR spectroscopy on purified enzyme, they claim to show that the spectrum for Fe nitrogenase with added Mo is similar to that of the Mo nitrogenase from the wild-type, so it seems like the FeMo cofactor is incorporated into the Fe nitrogenase. I would've liked to see their result for the Fe nitrogenase without added Mo as a control, but I'll have to take their word for it. Though they did do metal analysis that found ratios of Fe to Mo similar to that of the Mo nitrogenase.

The fact that chloramphenicol didn't prevent the Mo effect seemed to show that the proteins required to make FeMoco were present before Mo was added. This was confirmed with lacZ fusions to related genes to observe expression more directly.

What This Means
Is FeMoco actually replacing FeFeco in completed enzymes? Seems more likely that FeMoco is inserting into incomplete apoprotein, but it's hard to distinguish between these possibilities. Considering that the process seems to continue over several days, maybe the FeMoco is actually displacing FeFeco from completed proteins over time. This is supported by the observation of oxygen enhancement of the Mo effect; oxygen seems to make the enzyme more labile.

Reference:

Friday, August 7, 2015

555 - Differential Effects on N2 Binding and Reduction, HD Formation, and Azide Reduction with α-195His- and α-191Gln-Substituted MoFe Proteins of Azotobacter vinelandii Nitrogenase

Similar to 554, this study looked at mutated versions of the Mo nitrogenase in Azotobacter vinelandii, but different reactions this time: interactions with nitrogen gas (hooray), with dihydrogen and dideuterium, and with azide (N3-).

What They Saw
Adding nitrogen gas revealed that Asn 195 couldn't fix nitrogen; Gln 195 had a slight ability. However, replacing argon with pure nitrogen reduced hydrogen production by Asn 195 about 30%, though increasing the pressure with additional nitrogen didn't affect things further except maybe to increase the ATP required for each electron transfer, almost double what it is at 100% argon. Nitrogen didn't seem to affect hydrogen from Lys 191 at all.

Adding nitrogen also inhibited acetylene reduction in Asn 195, 28%; and again, did not inhibit Lys 191. For the former, nitrogen seems to inhibit the reaction competitively but reversibly; removing the nitrogen restored the rate almost to what it had been.

They tried adding hydrogen to acetylene reduction assays with Asn 195, enough to raise the pressure to two atmospheres. This didn't affect anything with argon; ethylene and ethane were both produced about the same amount. But when nitrogen was present, hydrogen restored most or all the activity that nitrogen would've inhibited.
They also saw that having 50% deuterium with the rest nitrogen doesn't really result in inhibition of hydrogen production by Asn 195.

When they tried adding sodium azide, this didn't really affect hydrogen production, but the activity reducing it to ammonia or hydrazine (N2H4) was much less for all the mutants than for the wild-type, at least 8x less. Adding carbon monoxide to Asn 195 assays abolished any azide reduction activity, but adding hydrogen had no effect. The azide might've reduced electron flux through Asn 195 a little (20%) but CO prevented this reduction too.

What This Means
Even some of the mutants that can't fix nitrogen seem to interact with it to some extent, as evidenced by its inhibiting other reactions. The other findings are pretty interesting too. It all relates to how the mutations affect the activity: in affinity, in substrate fit in the active site, and in electron flux through the whole complex.

Reference:

Thursday, August 6, 2015

554 - Azotobacter vinelandii Nitrogenases Containing Altered MoFe Proteins with Substitutions in the FeMo-Cofactor Environment: Effects on the Catalyzed Reduction of Acetylene and Ethylene

This is another study looking at mutating the Mo nitrogenase protein in Azotobacter vinelandii to see how it changes the enzyme's activity. Here's a picture they gave of the active center, with the FeMo cofactor in the middle and the protein surrounding it, with certain amino acids they were targeting:

What They Saw
They targeted conserved amino acids, common to homologs in many organisms, such as Gln 191 and His 195, with three mutations: Lys 191 (seen before in 536), Asn 195, and Gln 195. These proteins were extracted and purified.

Under argon, Gln 195 produced about as much hydrogen as the wild-type, while the others only about half as much (though apparently they only contained half as much FeMo cofactor). Under 10% acetylene, the wild-type put most electrons toward producing ethylene, while the others only devoted 55% at most, the others going to hydrogen (Gln 195) or hydrogen and ethane (others).

The mutations also affected how the protons were added to acetylene, whether in cis or in trans, as determined by using C2D2 instead of C2H2 and looking at where there was hydrogen or deuterium. The mutants had higher proportions of trans-C2D2H2 compared to the wild-type, except Gln 195 which had lower. This seems related to their ability to reduce acetylene all the way to ethane or not.

Then they tested whether ethylene instead of acetylene, with or without CO or acetylene too, could be a substrate or inhibitor of activity. Having 50% ethylene with the rest argon led to ethane production from all versions, including the wild-type, except not Lys 191. It seemed to inhibit overall flux a little, though for most versions it didn't inhibit as much when CO was present (the exception was Lys 191, which had about 3x less flux with CO present compared to just argon, though in both cases it all went to hydrogen). Interestingly, adding 50% hydrogen with 50% ethylene increased the amount of ethane for all versions. Strangely, though it didn't reduce ethylene to ethane, adding 10% acetylene to the mix with Lys 191 showed some ethane production; so it doesn't reduce ethylene, only acetylene. Acetylene also increased the rate of ethane production with Asn 195.

What This Means
This helps understand the precise reaction that takes place in nitrogenase. It seems like the affinity of the enzyme for the substrate affects how far that substrate is reduced before being replaced by a fresh molecule. Still, it's hard to make real comparisons from in vitro assays, but I don't know that there's a good alternative.

Reference:

Wednesday, August 5, 2015

544 - Nitrogenase from vanadium-grown Azotobacter: Isolation, characteristics, and mechanistic implications

It was known that Mo seemed important for nitrogen fixation in Azotobacter vinelandii. This study looked at substituting V for Mo for nitrogen fixation. It wasn't discovered until about a decade later that there were two separate sets of genes for two versions of nitrogenase with different metals, so here they thought it was a substitution of metals in the same protein.

What They Saw
They grew A. vinelandii OP (aka CA) with Mo or V and extracted and purified its nitrogenase. They said it didn't grow without either Mo or V, which seems weird because it should be able to grow with just iron.

The V nitrogenase activity (measured as hydrogen production) was lower than that of the Mo nitrogenase, about 22% of it, though it's hard to compare in vitro assays. It also seemed less stable and more prone to heat inactivation.

They did detect traces of Mo in the V purification, so it's not clear exactly what's happening. There was about 20x more V than Mo. Both purifications could reduce acrylonitrile, propionitrile, and acetonitrile in addition to the more familiar substrates, and hydrogen was produced at the same time. Hydrogen inhibited nitrogen reduction and carbon monoxide inhibited everything except hydrogen production.

In terms of efficiency, they observed that the Mo nitrogenase allocated 70% of its electrons to nitrogen and only 30% to hydrogen (similar to the typical 75%/25% numbers), while electrons in the V nitrogenase only went to nitrogen 25% of the time, which works out to 6 electrons making 2 ammonia, and another 18 making 9 hydrogen. Other substrates gave different numbers, but the V nitrogenase always had higher flux to hydrogen.

It seemed like CO inhibition of the V version was competitive but it wasn't clear that the same was true of the Mo version. And acrylonitrile reduction was different between them: V nitrogenase produced about twice as much propane as opposed to propylene compared to the Mo version.

What This Means
It seems, from the results, pretty likely that they were studying the V nitrogenase (Vnf) in this study, despite slight contamination with Mo. The CO inhibition pattern, and other activity patterns, support this conclusion.

I didn't know that acrylonitrile and such could be substrates for nitrogenase, but perhaps the cyanide residue is reduced to make it a hydrocarbon, either propylene or propane. Not sure this seems more useful than other substrates.

Overall, it's interesting how much this study revealed that wasn't really known until later studies confirmed it.

Reference:
Burns, R. C., Fuchsman, W. H. & Hardy, R. W. F. Nitrogenase from vanadium-grown Azotobacter: Isolation, characteristics, and mechanistic implications. Biochem Biophys Res Commun 42, 353–358 (1971).

Tuesday, August 4, 2015

542 - Purification and Characterization of the vnf-encoded Apodinitrogenase from Azotobacter vinelandii

This study looks at the vanadium nitrogenase apoprotein and how it works with its own cofactors or those of the other versions.

What They Saw
Azotobacter vinelandii strains possessing or lacking genes for one or more of the nitrogenases were grown and their protein was extracted. They also extracted central cofactors for each type separately.

Without nifB, a strain can't produce any of the cofactors, so it's easier to get apoproteins. They took vanadium aponitrogenase (Vnf version) and activated it with FeVco. Not surprisingly, they only saw activity in strains containing vnf when vanadium was present and Mo or ammonium was not. Extracts of active strains lost activity when treated with heat or exposed to air for 45 minutes, though the holoenzyme seemed more stable to heat.

When they purified the enzyme as much as possible, the delta subunit (vnfG-encoded) seemed only loosely attached to the others, unlike in A. chroococcum where it purifies together with the others; though it's not clear that conditions were the same. It was necessary for active enzyme though, and seems to be involved in inserting the cofactor into the enzyme, but also something else.

They tried replacing FeVco with FeMoco in the V nitrogenase (or vice versa in the Mo version). With the V version and FeVco, carbon monoxide inhibited about 70% of the acetylene reduction activity but no hydrogen production activity. The Mo nitrogenase with FeVco had a fraction of the V version's acetylene activity but no nitrogen fixation; sadly they didn't test hydrogen production.

With the V nitrogenase and FeMoco, it had a bit (1/6th) of the acetylene reduction activity, which seemed insensitive to CO. Ethane production was proportionally higher (1:4 instead of 1:12). Hydrogen production was reduced about 40%. Nitrogen fixation was pretty much abolished.

With the correct cofactors, the V nitrogenase had about 31% the acetylene reduction activity, 34% of the hydrogen production activity, and 22% of the nitrogen fixation activity of the Mo nitrogenase, but it's not clear if these in vitro assays allow for accurate comparisons.

It didn't seem like FeFeco allowed any activity in the V aponitrogenase.

What This Means
It seems like nitrogen fixation requires a very specific environment, and messing with it in various ways (mutations, different cofactors) messes it up while allowing the enzymes to still do less strict activities.

Reference:
Chatterjee, R., Allen, R. M., Ludden, P. W. & Shah, V. K. Purification and Characterization of the vnf-encoded Apodinitrogenase from Azotobacter vinelandii. J. Biol. Chem. 271, 6819–6826 (1996).

Monday, August 3, 2015

541 - Diversity of Nitrogenase Systems in Diazotrophs

This review looks at different kinds of nitrogen-fixing enzymes, real and theoretical. Azotobacter vinelandii itself has three genetically distinct versions, with different central metals in their central cofactors: molybdenum, vanadium, and iron.

The molybdenum version is most common in nature, and used preferentially in organisms that possess it. It has also been studied the most. No known organism possesses either of the other two versions while lacking this one. Protein sequences of this enzyme in different organisms are remarkably similar to each other. It uses 2 ATP to transfer one electron from the dinitrogenase reductase protein to the dinitrogenase complex, and 6 such transfers reduce one dinitrogen to two ammonia (along with 2 electrons going to hydrogen).

The other versions were discovered when Mo or its enzyme were unavailable yet nitrogen fixation continued. They have an extra subunit of unknown function (perhaps cofactor insertion), but otherwise seem pretty similar in structure and function.

Other than those three, Streptomyces thermoautotrophicus has a novel system: it has Mo in the dinitrogenase and the dinitrogenase reductase equivalent is a manganese-superoxide oxidoreductase with no iron or sulfur. It doesn't do acetylene reduction, but far from being oxygen-sensitive, it depends on oxygen for its activity. The overall stoichiometry is similar to the Azotobacter Mo nitrogenase though, including the hydrogen production, but the minimum ATP requirement is only 4, instead of 16.

Then the authors go into some discussion of other nitrogenases, with the same apoenzymes as those in A. vinelandii but with different central cofactors that may or may not be found in nature. For example, a tungsten-iron cofactor, which has been studied before: it doesn't permit nitrogen fixation, but some proton reduction is possible. Then there were chromium- or manganese-iron cofactor proteins: when extracts were treated with o-phenanthroline and purified as apoproteins, activity could be partially restored with solutions including salts of Mn, V, Mo, Cr, or Re. W failed to give this effect, but the others permitted some acetylene and proton reduction activity. I don't have access to the citations for these claims though.

These in vitro proteins, even if they contain weird metals, also seem to contain Mo in equal proportions to the others. A. vinelandii UW3 lacks nif genes required for the primary nitrogenase, but can use alternatives, and some suggest that the V nitrogenase may use Re or Mn or Cr instead; or maybe the Mo nitrogenase proteins are expressed but use these metals. It doesn't seem very clear, but it's interesting.

Reference:
Zhao, Y., Bian, S.-M., Zhou, H.-N. & Huang, J.-F. Diversity of Nitrogenase Systems in Diazotrophs. J Integr Plant Biol 48, 745–755 (2006).